Coding

Part:BBa_K209088:Design

Designed by: Ryan Quan   Group: iGEM09_UCSF   (2009-10-14)


ACA-linker-RasGEFR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1408
    Illegal EcoRI site found at 5145
    Illegal EcoRI site found at 5350
    Illegal XbaI site found at 3922
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1408
    Illegal EcoRI site found at 5145
    Illegal EcoRI site found at 5350
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1408
    Illegal EcoRI site found at 5145
    Illegal EcoRI site found at 5350
    Illegal BglII site found at 3145
    Illegal BglII site found at 4074
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1408
    Illegal EcoRI site found at 5145
    Illegal EcoRI site found at 5350
    Illegal XbaI site found at 3922
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1408
    Illegal EcoRI site found at 5145
    Illegal EcoRI site found at 5350
    Illegal XbaI site found at 3922
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4957


Design Notes

Aar1 AB BC CD


Source

plasmid

References